Enhancing permanganate chemiluminescence detection for the determination of glutathione and glutathione disulfide in biological matrices
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منابع مشابه
Enhancing permanganate chemiluminescence detection for the determination of glutathione and glutathione disulfide in biological matrices.
Acidic potassium permanganate chemiluminescence enables direct post-column detection of glutathione, but its application to assess the redox state of a wider range of biological fluids and tissues is limited by its sensitivity. Herein we show that the simple on-line addition of an aqueous formaldehyde solution not only enhances the sensitivity of the procedure by two orders of magnitude, but al...
متن کاملDetermination of intracellular glutathione and glutathione disulfide using high performance liquid chromatography with acidic potassium permanganate chemiluminescence detection.
Measurement of glutathione (GSH) and glutathione disulfide (GSSG) is a crucial tool to assess cellular redox state. Herein we report a direct approach to determine intracellular GSH based on a rapid chromatographic separation coupled with acidic potassium permanganate chemiluminescence detection, which was extended to GSSG by incorporating thiol blocking and disulfide bond reduction. Importantl...
متن کاملDetermination of intracellular glutathione and glutathione disulfide using high performance liquid chromatography with acidic potassium permanganate
This is the published version: McDermott, Geoffrey P., Francis, Paul S., Holt, Kayla J., Scott, Kristen L., Martin, Sheree D., Stupka, Nicole, Barnett, Neil W. and Conlan, Xavier A. 2011, Determination of intracellular glutathione and glutathione disulfide using high performance liquid chromatography with acidic potassium permanganate chemiluminescence detection, Analyst, vol. 136, no. 12, pp. ...
متن کاملDetermination of glutathione disulfide levels in biological samples using thiol-disulfide exchanging agent, dithiothreitol.
A reverse-phase HPLC method incorporating dithiothreitol (DTT) reduction for quantitative determination of oxidized glutathione (GSSG) in biological samples is described here. This method is based on our previous enzymatic reduction technique that uses N-1-(pyrenyl) maleimide (NPM) as a derivatizing agent. In our earlier method, glutathione disulfide (GSSG) was measured by first reducing it to ...
متن کاملGlutathione disulfide liposomes – A research tool for the study of glutathione disulfide associated functions and dysfunctions
Glutathione disulfide (GSSG) is the oxidized form of glutathione (GSH). GSH is a tripeptide present in the biological system in mM concentration and is the major antioxidant in the body. An increase in GSSG reflects an increase in intracellular oxidative stress and is associated with disease sates. The increase has also been demonstrated to lead to an increase in protein S-glutathionylation tha...
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ژورنال
عنوان ژورنال: The Analyst
سال: 2014
ISSN: 0003-2654,1364-5528
DOI: 10.1039/c4an00255e